CXCR1 and CXCR2 mRNA is expressed in human neutrophils and lymphocytes but not in human eosinophils. Human neutrophil, lymphocyte, and eosinophil mRNA was isolated and first-strand cDNA synthesis was performed. PCR was performed with primer pairs specific for CXCR1 (expected size, 507 bp), CXCR2 (expected size, 520 bp), CD52 (expected size, 385 bp), and CD16 (expected size, 297 bp). The amplicons were separated by electrophoresis in 1.8% agarose gel and stained by ethidium bromide. Lane M, 100-bp DNA size marker; lane N, human neutrophil mRNA; lane L, human lymphocyte mRNA; lane E, human eosinophil mRNA. One representative experiment of four performed is shown.