Effects of anti–SDF-1 antibody on chemotactic activity. FDCP-2 cells were placed in the upper chambers of Transwell plates with or without anti–SDF-1 antibody at the indicated concentrations. Six hundred microliters of STR-2 cell-conditioned medium was placed in the lower chambers. After 4 hours of incubation, transmigrated cells into the lower chambers were counted under an inverted microscope. Results were shown as the mean ± SD of data obtained from three different experiments.