Fig. 3.
Phenotype of adherent and nonadherent DC subsets from mesenteric and peripheral LN. Mesenteric and peripheral LN DC were purified as described, incubated for 90 minutes at 37°C, and the nonadherent and adherent fractions collected and analyzed for the expression of CD8 and LFA-1. The contour plots show the LFA-1 versus CD8 profiles of adherent and nonadherent DC after gating for CD11c+ cells. The dotted lines mark the lower limit defining the expression at high levels of the corresponding marker. These data are representative of four experiments with similar results.