Analysis of LC migrating to the draining LN after skin sensitization. BALB/c mice received 10μL of 1% FITC in ADBP on the dorsum of both ears and after 5 days the DC from the draining auricular LN were purified and analyzed. Contour plots show the CD11c versus CD8 profile of purified auricular LN DC, and the correlation between CD8 expression and FITC staining within these cells. FITC ⁺cells were only found within the CD8 ^int DC subset and represented around 20% of total auricular LN DCs. Histograms show the phenotype of CD8 ^int FITC ⁺ DC (3) compared with that of CD8 ^high FITC ^- DC (1) and CD8^int FITC ^- DC (2). The vertical dotted lines mark the lower limit defining the expression at high levels of the corresponding marker. These data are representative of four experiments with similar results.