Fig. 4.
Phosphorylation of ERK1, ERK2, and p38 MAPK in human neutrophils stimulated by TNF. Cells were stimulated with indicated concentrations of TNF for 10 minutes at 37°C or stimulated with 100 U/mL TNF for indicated periods at 37°C. Phosphorylation of ERK1, ERK2 (upper 2 panels), and p38 MAPK (lower 2 panels) was analyzed by immunoblotting using antibody against phosphorylated form of each protein. The cell lysates equivalent to 3 × 106 cells were loaded onto each lane. The results shown are representative of three independent experiments.
