Study of Mpl-L transcripts by a sensitive fluorescent RT-PCR assay. Mpl-L transcripts were amplified from total RNA of different cell types, and the amplification products were run on a sequencing gel. Size of the amplified product is 183 bp. Lane 1, HepG2 diluted 1:10; lane 2, HEL; lane 3, CD34+ cells from peripheral blood cytapheresis; lane 4, cultured MKs from a normal marrow; lane 5, spontaneously growing MKs from t10; lane 6, spontaneously growing MKs from t11; lane 7, spontaneously growing MKs from t12; lane 8, CD34+ from t16; lane 9, spontaneously growing MKs from t16; lane 10, CD34+ from t19; lane 11, spontaneously growing MKs from t19; lane 12, blood monuclear cells from m1; lane 13, CD34+ cells from m1; lane 14, spontaneously growing MKs from m1; lane 15, CD34+ from m2; lane 16, spontaneously growing MKs from m2; lane 17, CD34+ from m3; lane 18, spontaneously growing MKs from m3; lane 19, cultured MK after PEG-rhuMGDF stimulation from m3; lane 20, CD34+cells from m7; lane 21, spontaneously growing MKs from m6; lane 22, cultured MK after PEG-rhuMGDF stimulation from m6; lane 23, spontaneously growing MKs from m8; lane 24, cultured MK after PEG-rhuMGDF stimulation from m8; lane 25, CD34+ cells from m4; lane 26, spontaneously growing MKs from m4; lane 27, H2O control; lane 28, H2O control.
