Fig. 1.
HSV amplicon vectors. Coding sequences for human B7.1 or human RANTES were cloned downstream of the HSV IE4/5 promoter in pHSVPrPUC amplicon plasmid as indicated. HSVlac, which places theEscherichia coli β-galactosidase gene (lacZ) under the transcriptional control of the IE 4/5 promoter, has been described previously.19 Ampr denotes location of the ampicillin resistance gene. OriS represents HSV-1 replication origin and Pac is the HSV-1 cleavage and packaging sequence.