The effect of MoAb treatment on the percentage of granulocytes in peripheral blood after croton oil application to the ears. Blood for FACS analysis was collected at indicated time points after the application of croton oil and injection of antibody. (A) L-selectin–deficient mice: Mice treated with combined endothelial selectin antibodies have significantly increased neutrophils at each posttreatment time point (P < .03 compared with control). By 20 hours, the percentage of circulating neutrophils is decreasing in all animals. (B) Wild-type mice: combined anti-endothelial selectin antibody increases the percentage of circulating granulocytes at each time point (P < .005 compared with control). Anti–L-selectin treatment does not significantly affect circulating granulocyte percentages.