Antibody blocking of a CHS ear swelling response to DNFB. Mice were treated as described and ears were measured 24 hours after elicitation. (A) Wild-type mice: combined anti-endothelial selectin antibody significantly reduces antigen-specific ear swelling compared with both untreated and anti-CD45R_B control treated animals. Antibody to VLA-4 is equally effective, whereas neither anti–L-selectin nor anti-CD44 treatment has any effect. (B) L-selectin–deficient mice: treatment with combined anti-endothelial selectin or anti–VLA-4 significantly reduces ear swelling. Anti-CD44 and control anti-CD45R_B have no effect. n ≥ 4 for each treatment group. *P < .001 compared with both untreated and anti-CD45R_B control treated animals.