American Society of Hematology

Fig. 1.

$Fig. 1. Primary breast cancer cell cultures. (A) Cells contained in an ascites (upper panel) and a pleural effusion sample (lower panel) from 2 representative breast cancer patients were cultured in -MEM/10% FCS. At the indicated time points, numbers of CK-positive cells (•) and CK-negative cells (○) were determined by immunocytochemical analysis as described in Materials and Methods. At the time points indicated with an asterisk (*), detection of Ber-Ep4 was also performed yielding nearly identical cell numbers as CK-positive cells. Cultures were maintained by subcultivating total cells, ie, adherent and nonadherent fractions. (B) Immunostaining with anti-CK MoAbs of the cultures shown in the upper panel of (A) at the indicated time points (original magnification, ×100).$

Primary breast cancer cell cultures. (A) Cells contained in an ascites (upper panel) and a pleural effusion sample (lower panel) from 2 representative breast cancer patients were cultured in -MEM/10% FCS. At the indicated time points, numbers of CK-positive cells (•) and CK-negative cells (○) were determined by immunocytochemical analysis as described in Materials and Methods. At the time points indicated with an asterisk (*), detection of Ber-Ep4 was also performed yielding nearly identical cell numbers as CK-positive cells. Cultures were maintained by subcultivating total cells, ie, adherent and nonadherent fractions. (B) Immunostaining with anti-CK MoAbs of the cultures shown in the upper panel of (A) at the indicated time points (original magnification, ×100).

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