Expression of the VE-cadherin −2486CAT fusion gene in various cell lines. Construct −2486CAT and pGL3 plasmid were cotransfected in cells of epithelial (HeLa), hepatocytic (HepG2), or erythro-megakaryocytic origin (HEL and Lin175). The HSVTK (see Fig 1) or the Rous sarcoma virus (RSV) promoters was used as positive control. CAT activities were determined with the equivalent of 150, 3,000, 600, or 200 light units of extracts from HeLa, HepG2, HEL, or Lin175, respectively. Data are the average of 3 independent experiments.