Multilineage reconstitution of recipient mice transplanted with the fetal liver cells ofjmj^trap/trap embryos. Strategy of reconstitution analysis (A). Lethally irradiated recipient mice were injected with the fetal liver cells of jmj^trap/trap andjmj^+/trap and after 1 month, the presence of donor-derived cells was determined by PCR. The representative results of genotype determined by PCR analysis specific forjmj^trap allele (B-E) and wild-type jmjallele (F through I). The recipients transplanted with the fetal liver cells of jmj^+/trap embryos (B, D, F, and H) andjmj^trap/trap embryos (C, E, G, and I) were examined. BM, SP, and TH indicate bone marrow, spleen, and thymus, respectively. TER, Gr, B220, and Thy indicate TER⁺, Gr-1⁺, B220⁺, and Thy-1⁺cells, respectively. TER⁺ and Gr-1⁺ cells were isolated from the bone marrow. B220⁺ and Thy-1⁺ cells were from spleen and thymus, respectively.