Quantitative assessment of Ad vector-mediated GFP expression in murine alveolar macrophages in vitro. Shown is flow cytometry analysis of alveolar macrophages infected for 48 hours in suspension with AdGFP at 10, 50, and 200 moi. Infection withAdNull is used as control. Shown on the ordinate is cell number and on the abscissa is shown the intensity of GFP. (A) AdNull, 200 moi. (B) AdGFP, 10 moi. (C) AdGFP, 50 moi. (D) AdGFP, 200 moi.