Time course of expansion in numbers of alveolar macrophages after in vitro infection of alveolar macrophages by AdmGM-CSF and subsequent transfer of the genetically modified AM to the lung. Alveolar macrophages were infected in vitro with AdmGM-CSF (AM GM-CSF) or AdNull (AM Null) at 100 moi. After 90 minutes, the cells were washed and 2 × 105 AM were transplanted by the intratracheal route to the lungs of syngeneic mice. (A) Total number of cells recovered by lavage 7 days after the administration of genetically modified AM. Shown are total numbers of AM, granulocytes, and lymphocytes. The granulocytes were greater than 99% neutrophils. (B) Number of AM recovered by lavage 1 and 5 weeks after administration of AM genetically modified in vitro with AdmGM-CSF compared with AdNull or naive mice. The data regarding the number of AM from 1 week are the same as the AM data from (A). The data represent the mean ± standard error of the mean for 4 to 6 mice for each data point.
