Analysis of B12SPM labeling of hematopoietic stem- and progenitor-enriched cell populations. (A) and (C) depict the Lin⁻/FSC^low/c-Kit⁺ stem cell–enriched population, whereas (B) and (D) depict the Lin⁻/FSC^high/c-Kit⁺ progenitor cell–enriched population. (A) and (B) shaded area, ASMKO cells; open area, normal cells. (C) and (D) shaded area, nontransduced ASMKO cells; open area, transduced ASMKO cells. Note that FL-1 measures B12SPM. The experiment was repeated three times, and the representative data from one experiment are shown.