RT-PCR analysis. Each RNA was transcribed with (+; odd lanes) or without (−; even lanes) RT. The PCR products were transferred to a nylon membrane and analyzed by hybridization with end-labeled internal oligonucleotide probes specific for each gene. RNAs were evaluated for the UOC-B1 (lanes 1 and 2) and HAL-01 (lanes 3 and 4) t(17;19)-positive human leukemic cell lines, patient samples with t(17;19)-positive ALL (lanes 5 through 8), the t(17;19)-negative Nalm6 early B-lineage leukemic cell line (lanes 9 and 10), 697 pre-B leukemic cell line (lanes 11 and 12), and Jurkat T-cell leukemic cell line (lanes 13 and 14), as well as a control PCR reaction lacking RNA template (lanes 15 and 16).