(A) Synthesis and secretion of SRPUL-CF. Metabolically labeled lysates of FL5.12 (SRPUL-CF)3 cells were immunoprecipitated with the -FLAG MoAb (M2) (lanes 3 and 4) and compared with control FL5.12 (pMT) cells (lanes 1 and 2). (B) Secretion of SRPUL-CF by FL5.12 (SRPUL-CF)3 cells (lane 4), as shown by immunoprecipitation from the culture medium 16 hours after the addition of zinc (100 μmol/L).