Effects of E2A-HLF, ANNEXIN VIII, and SRPUL-CF on the survival of FL5.12 cells deprived of growth factor. (A) Immunoblot analysis with antisera to HLF(C) and ANNEXIN VIII and with the -FLAG MoAb (M2). Results are shown for three independently derived clones of G418-resistant FL5.12 cells transfected with each of the full-length cDNAs (FL5.12 [E2A-HLF], FL5.12 [ANNEXIN VIII] and FL5.12 [SRPUL-CF]) under control of the zinc-regulated pMT promoter as well as controls transfected with the empty vector (FL5.12 [pMT]). (B) Survival of FL5.12 cells expressing E2A-HLF, ANNEXIN VIII, or SRPUL-CF, in the absence of IL-3. Cells growing exponentially in IL-3–supplemented media for 16 hours in the presence or absence of zinc were adjusted to 5 × 10⁵ cells/mL on day 0, and cultured after removal of IL-3. The cell number of viable cells is shown for E2A-HLF-, ANNEXIN VIII-, and SRPUL-CF–expressing transfected FL5.12 clones grown in the presence (open symbols) or absence (closed symbols) of zinc (100 μmol/L).