STAT activation from mice with a targeted deletion in the G-CSF-R derived from SCN. (A) EMSA of nuclear extracts from bone marrow cells of homozygous WT (wt/wt) and mutant “knock-in” (▵715/▵715) mice, as described in Fig 2A, except that both m67 and β-cas oligonucleotides were used, as indicated. The position of STAT1 (S1), STAT3 (S3), STAT5 (S5), and nonspecific (n.s.) complexes are indicated. (B) EMSA of nuclear extracts from bone marrow cells from homozygous WT (wt/wt) and mutant “knock-in” (▵715/▵715) mice with m67 oligonucleotide, as described in Fig 2A, except cells were incubated for 15 minutes with the indicated concentrations of G-CSF. (C) Dose response of STAT3 activation from WT (filled squares) and “knock-in” (open triangles) mice. Quantitative analysis of EMSAs performed as described in (B), setting maximal STAT3 activation at 100%, and basal activation at 0%. The graph shows the mean and range of two independent experiments.