Nucleotide and predicted amino acid sequence of human cellubrevin. (A) Nucleotide and amino acid sequence of Hceb. The numbers correspond to the number of nucleotides and the underlined regions correspond to the sequences for which degenerate primers were designed. The sequence of the Hceb clone was submitted to Genbank, accession number U64520. (B) Comparison of the Hceb amino acid sequence to other human VAMPs and rat cellubrevin. The dots represent identical amino acids. The underlined region represents the peptide that was used to make a specific antihuman cellubrevin Ab. The bold methionine (M) represents the VAMP 2 vesicle targeting signal, which is also present in Hceb.