Fig. 2.
![Fig. 2. Human cellubrevin is expressed in many human tissues. The Northern blot containing 2 μg of poly A+ mRNA from the indicated human tissues was incubated with a [32P] nick-translated DNA probe made from the 292-bp of the 3′ untranslated region of human cellubrevin (bp 402-693, Fig 1A). The blot was washed and exposed to film for 18 hours at −80°C with an intensifying screen. The positions of the size standards in kilobases are shown at right. The same blot was also probed with a [32P] nick-translated β-actin DNA probe, washed, and exposed to film at −80°C for 2 hours. Some tissues contain two isoforms that will hybridize with the β-actin probe (pancreas and placenta).](https://ash.silverchair-cdn.com/ash/content_public/journal/blood/93/2/10.1182_blood.v93.2.571/4/blod40205002w.jpeg?Expires=1735545723&Signature=QF4EGpTzlAsfW--06jRE~AVrBt4ezzbwlnXmiOlxSo4cVYeJZf0GTOHk0~o4jyM34axuVXAaSajZ74nf5-rS6Wxx0WN9uEXBACEUNUyyp~knxBCvlfbkX1EYUNgPs4QaPt2G1QZLBY6KPHIrZXHYpyHD7sS5o6CJs5mcZd9oKAjtNlZObK6aCmvqKTLY5j945870rQ~Ep9N5TyYwZ3ZfwlnTuKTexFzQ5reaOhSUXq2oT-~LiXr49f44bBY4H2v9rSBFxH2OYLvQ9t2q-6nnbmfsl~lU0CfuaYL5-YvVjj6mF6bJK0gpgQxJ4MYn~EN0IGiUyPcGVm4eJQilusysOw__&Key-Pair-Id=APKAIE5G5CRDK6RD3PGA)
Human cellubrevin is expressed in many human tissues. The Northern blot containing 2 μg of poly A+ mRNA from the indicated human tissues was incubated with a [32P] nick-translated DNA probe made from the 292-bp of the 3′ untranslated region of human cellubrevin (bp 402-693, Fig 1A). The blot was washed and exposed to film for 18 hours at −80°C with an intensifying screen. The positions of the size standards in kilobases are shown at right. The same blot was also probed with a [32P] nick-translated β-actin DNA probe, washed, and exposed to film at −80°C for 2 hours. Some tissues contain two isoforms that will hybridize with the β-actin probe (pancreas and placenta).
