Fig. 3.
Antihuman cellubrevin antibody does not cross-react with VAMP 1 or VAMP 2. (A) E. coli cells expressing His6-tagged VAMP 1, 2, and Hceb constructs were induced with 1 mmol/L IPTG for 4 hours to produce each recombinant protein. Bacterial extracts from the minus and plus IPTG-induced samples were separated on a 12.5% SDS-PAGE gel and proteins were stained with Coomassie Brilliant Blue. Lanes 1 and 2, VAMP 1 ± IPTG; lanes 3 and 4, VAMP 2 ± IPTG; lanes 5 and 6, Hceb ± IPTG. (B) The same samples were separated by SDS-PAGE and then transferred to nitrocellulose and blotted with a specific anti-Hceb Ab at a concentration of 1/1,000.
