Fig. 7.
Fig. 7. Human cellubrevin staining remains intracellular in surface-activated platelets. Platelets were prepared as resting (see Materials and Methods) or surface-stimulated in the presence of 10 μmol/L CaCl2 or 10 μmol/L CaCl2 and 0.1 U/mL thrombin. Platelets were fixed with 3.7% formaldehyde and permeabilized with 100 μg/mL digitonin. Left panel, anti-gpIIβ/III antibody (1/100), Texas Red-conjugated antimouse antibody (1/150) (gpIIβ/III); anti-Hceb antibody (1/25), FITC-conjugated antirabbit antibody (1/150) (Hceb). Middle panel, anti-gpIIβ/III antibody (1/100), Texas Red-conjugated antimouse antibody (1/150) (gpIIβ/III); antiserotonin antibody (1/10), FITC-conjugated antirabbit antibody (1/150) (serotonin). Right panel, anti-gpIIβ/III antibody (1/100), Texas Red-conjugated antimouse antibody (1/150) (gpIIβ/III); anti-vWF antibody (1-500), FITC-conjugated antirabbit antibody (1/500) (vWF). Original magnification 2,800×.

Human cellubrevin staining remains intracellular in surface-activated platelets. Platelets were prepared as resting (see Materials and Methods) or surface-stimulated in the presence of 10 μmol/L CaCl2 or 10 μmol/L CaCl2 and 0.1 U/mL thrombin. Platelets were fixed with 3.7% formaldehyde and permeabilized with 100 μg/mL digitonin. Left panel, anti-gpIIβ/III antibody (1/100), Texas Red-conjugated antimouse antibody (1/150) (gpIIβ/III); anti-Hceb antibody (1/25), FITC-conjugated antirabbit antibody (1/150) (Hceb). Middle panel, anti-gpIIβ/III antibody (1/100), Texas Red-conjugated antimouse antibody (1/150) (gpIIβ/III); antiserotonin antibody (1/10), FITC-conjugated antirabbit antibody (1/150) (serotonin). Right panel, anti-gpIIβ/III antibody (1/100), Texas Red-conjugated antimouse antibody (1/150) (gpIIβ/III); anti-vWF antibody (1-500), FITC-conjugated antirabbit antibody (1/500) (vWF). Original magnification 2,800×.

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