CCR-1 and CCR-3 regulate the migratory capacity of monocyte-derived DCs. Monocyte-derived DCs (10⁶) were pretreated with the indicated concentrations of anti–CCR-1 MoAb, anti–CCR-3 MoAb, or cont. IgG for 30 minutes at 37°C and seeded on the filters precoated on the lower surface with 5 μg of gelatin. (A) RANTES (1 μg/mL), (B) CM derived from unstimulated or PMA (50 ng/mL) plus IoM (500 ng/mL)-stimulated TCs, (C) CM derived from coculture of TCs and DCs, and (D) CM derived from culture of DCs used as a chemoattractant were added to the lower chamber. After 2 hours of incubation, the cells that migrated to the lower surface were visually counted.