MoAbs to CCR-1 and CCR-3 suppress capacity of monocyte-derived DCs to stimulate allogeneic TCs-derived IFN-γ secretion. TCs purified from PBMCs (10⁵) were cultured (A) with monocyte-derived DCs (10⁴) in the presence of the indicated concentrations of anti–CCR-1 MoAb, anti–CCR-3 MoAb, or cont. IgG or (B) with different numbers of monocyte-derived DCs in the presence of 10 μg/mL anti–CCR-1 MoAb, anti–CCR-3 MoAb, or cont. IgG. The IFN-γ secretion in the culture supernatants was measured by ELISA on day 5. Values are the mean ± SD obtained for triplicate cultures and are representative of those obtained in two individual experiments.