American Society of Hematology

Fig. 5.

$Fig. 5. Flow cytometric analysis for GATA-2irGFP or control irGFP vector expression in the Sca-1+ Lin−fraction of bone marrow cells. Twenty-four hours after completion of the transduction period with the indicated vectors, bone marrow cells were stained for expression of the Sca-1 and lineage-specific cell surface markers as described in Materials and Methods. The Sca-1+ Lin− cell subset (lower right quadrant of each dot plot) was gated on and GFP fluorescence in this cell subset is shown in the histogram below each dot plot. The percentages of GFP+ Sca-1+Lin− cells are indicated above each histogram. A separate experiment yielded similar results.$

Flow cytometric analysis for GATA-2irGFP or control irGFP vector expression in the Sca-1⁺ Lin⁻fraction of bone marrow cells. Twenty-four hours after completion of the transduction period with the indicated vectors, bone marrow cells were stained for expression of the Sca-1 and lineage-specific cell surface markers as described in Materials and Methods. The Sca-1⁺ Lin⁻ cell subset (lower right quadrant of each dot plot) was gated on and GFP fluorescence in this cell subset is shown in the histogram below each dot plot. The percentages of GFP⁺ Sca-1⁺Lin⁻ cells are indicated above each histogram. A separate experiment yielded similar results.

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