Levels of GATA-2irGFP– or control-vector irGFP-expressing cells in the peripheral blood of animals selectively transplanted with vector-expressing bone marrow cells. Flow cytometric histograms of GFP fluorescence are shown for the RBC, PLT, and WBC subsets of peripheral blood from representative animals selectively transplanted with control irGFP- or GATA-2irGFP–expressing bone marrow cells. The top three panels represent analyses performed 4 weeks after transplantation. The GATA-2irGFP 4 and GATA-2GFP 5 panels were obtained at 6 and 10 weeks after transplantation, respectively. Two additional transplanted GATA-2irGFP animals had less than 10% GFP⁺cells in all blood lineages at the 10-week time point, whereas all control GFP animals (n = 4) displayed at least 80% GFP⁺ cells in all three lineages at all time points analyzed. The GATA-2irGFP 1 animal shown above had the highest level of vector-expressing cells in the cohort of six transplanted GATA-2irGFP animals at the 4-week time point, with the other GATA-2irGFP animals showing levels similar to or less than those of the GATA-2irGFP 3 animal.