Autoantibody production after splenocyte transfer. Bm1 mice were transferred splenocytes from either C57BL/6 or MIP-1−/− B6 mice. Mice were bled from the lateral tail vein and 50 μL of blood was diluted in 1 mL of 4 mol/L citric acid. The serum was collected by centrifugation of the sample at 13,000g and frozen at −70°C. The serum was tested for the presence of IgG antichromatin autoantibodies using an enzyme-linked immunosorbent assay (ELISA). Control mice were C57BL/6 mice that received splenocytes from C57BL/6 mice.