Cytological characteristics of neutrophilic cells grown by SCF + TPO from CD34⁺ cord blood cells. Staining of cultured cells grown by SCF + TPO at 8 weeks with May-Grünwald-Giemsa (A); with POX (C); with a MoAb for MPO (D), and with ALP (E). The cells generated by SCF + TPO at 8 weeks were harvested and recultured with SCF + TPO + G-CSF. After 1 week, the cells were processed for staining with May-Grünwald-Giemsa (B), and with ALP (F). Original magnification: ×1,000 (A-D), ×400 (E,F).