Influence of IL-2, IL-7 (10 U), and IL-15 (10 U) on the DNA binding of Jun and Fos proteins in SeAx cells. (A) Lane 1, unstimulated SeAx cells; lane 2, IL-2 (100 U)-stimulated SeAx cells; lane 3, IL-7 (10 U)-stimulated SeAx cells; lane 4, IL-15 (10 U)-stimulated SeAx cells. (B) The IL-stimulated AP-1 oligomer-binding protein is JunD. Lane 1, peripheral blood lymphocytes (PBLs, negative control). Lanes 2 to 5, no IL; lanes 6 to 9, IL-7; lanes 10 to 13, IL-15. The individual DNA binding proteins were identified by specific antibodies. Lanes 1, 2, 6, and 10, no Ab; lanes 3, 7, and 11, c-Jun Ab; lanes 4, 8, and 12, c-Fos Ab; lanes 5, 9, and 13, Jun-D Ab. JunD-Ab, JunD-DNA-antibody complex; C1, JunD-DNA complex.