Fig. 1.
![Fig. 1. Pulse-chase labeling experiments in CHO cells transfected with pEDFVIIwt, pEDFVIICys97, and pEDFVIIArg100. After a 15-minute pulse with [35S] methionine, the cells were chased for 30, 60,120, 180, and 240 minutes. Equivalent amounts of cell lysate (top) or conditioned media (bottom) for FVIIwt (wt), FVIICys97(97) and FVIIArg100 (100) were immunoprecipitated using a Mab against FVII and analyzed by 8% SDS-PAGE under nonreducing conditions. The location of molecular weight markers in kD is denoted at the left-hand side of the figure. In the conditioned media (bottom), the 77 kD band was nonspecific as it was observed with equal intensity in untransfected CHO cells (data not shown).](https://ash.silverchair-cdn.com/ash/content_public/journal/blood/93/4/10.1182_blood.v93.4.1237/5/blod404020011w.jpeg?Expires=1735833509&Signature=J81Id0gY-iYz841MHP5AgKbGCl5gBODTKi91an14mqplHaLWt8VO0q5Cb756PycofvuXOpcZRCkRuyHLUDAvn02sMcq6VX~EJtuvjKoHPM2FdsZrltlssjWNHhISS9kcvy-59-u1bSmhEWl4xBtR06D~8hFrHUZsK9e4kPJlaPvlc0gcfMEF8MuymUa4XpURcIjPAqvc9X5neLC6MTXDvphVnDWFTIkK6Ulgs1-Jc43x-HArYnjTKCWsLlCuf0QcKWPo~0B28Mkv~unatSFQvZ5yUq48tcV8NtHdHo3zsLz9PKS1GGwkGIRe6rMeLOvgiO48i7QftzOFbcc~eLXO4Q__&Key-Pair-Id=APKAIE5G5CRDK6RD3PGA)
Pulse-chase labeling experiments in CHO cells transfected with pEDFVIIwt, pEDFVIICys97, and pEDFVIIArg100. After a 15-minute pulse with [35S] methionine, the cells were chased for 30, 60,120, 180, and 240 minutes. Equivalent amounts of cell lysate (top) or conditioned media (bottom) for FVIIwt (wt), FVIICys97(97) and FVIIArg100 (100) were immunoprecipitated using a Mab against FVII and analyzed by 8% SDS-PAGE under nonreducing conditions. The location of molecular weight markers in kD is denoted at the left-hand side of the figure. In the conditioned media (bottom), the 77 kD band was nonspecific as it was observed with equal intensity in untransfected CHO cells (data not shown).
