Western blot analysis of protein extracts made from wild-type and transgenic mice. (A) Total protein extracts were made from nontransgenic spleens (lanes 2 and 3) or from the spleens of transgene positive mice at age 2 months (lanes 4 and 5). Extracts made from individual spleen tumors from founder lines 36 and 37 are shown in lanes 6 and 7. Extracts made from a young transgenic mouse spleen activated with ConA and 50 U/mL rhIL-2 (CTL) versus 1,000 U/mL rhIL-2 (AdLAK), are shown in lanes 8 and 9. Extracts made from tumor line 37.4 and 37.29 are shown in lanes 10 and 11. (B) Total protein extracts derived from transgenic spleens or SCID spleens injected with wild-type spleen cells or tumor spleen cells are shown. An extract obtained from a tumor spleen derived from founder line 36 is shown in lane 2. Extracts from a SCID spleens injected 8 weeks earlier with wild-type spleen cells are shown in lanes 3 and 5. An extract from a SCID spleen injected with 1 × 108 cells from a line 36 tumor spleen is shown in lane 4, and SCID spleens injected with independent tumors from founder line 37 are shown in lanes 6 and 7. Note the presence of TAg in the SCID spleens from the animals injected with granzyme H-TAg splenic tumors.
