Flow cytometric analyses of cell lines. Dual-color flow analysis was performed with an antibody directed against TAg versus CD3 or CD8 (top panels) or with an antibody directed against CD16 versus NK1.1 (bottom panel). Note that all four cell lines analyzed are TAg⁺ NK1.1⁺. Cell lines from group A are CD3⁻ CD8⁻, those in group B are CD3⁺CD8⁻, those in groups C and D are CD3⁺ CD8⁺. Groups A, B, and C are CD16⁺, while the cells from group D are CD16⁻.