Production of CRP by human primary hepatocytes. Human primary hepatocytes were isolated from livers unsuitable for transplantation and cultured in the absence (control) or presence of IL-1β, IL-6, and a combination of IL-1β and IL-6 without (□) or with (▩) the addition of 10 ng/mL IL-4 for 18 hours. Levels of CRP were measured by ELISA in culture supernatants. Results are expressed as fold increase over unstimulated cells. Values represent the mean ± SEM of experiments performed with three donors. ^*P< .05 in comparison with cells cultured in the absence of IL-4.