Effect of CHX on GM-CSF–induced OSM secretion. PMN (10⁷/mL) were preincubated for 30 minutes with or without 10 μg/mL CHX and then stimulated with 100 U/mL GM-CSF for the times indicated; control cells were incubated with medium alone. The OSM secreted was assayed in the cell-free supernatants with an ELISA method. Results are expressed as the mean ± SEM of four independent experiments. #P < .05 compared with the control cells. *P < .05 compared with the CHX untreated cells. ND, not detected (<2.1 pg/mL).