Fig. 11.
Biological activity of OSM produced by PMN. HepG2 cells were incubated with the supernatant from LPS- plus GM-CSF–stimulated PMN (PMN conditioned medium [CMP]) or rhOSM (10 ng/mL); control cells (CTRL) were cultured in the medium alone. Anti-OSM antibodies were used as indicated. Secreted 1-acid glycoprotein (AGP) was determined in cell-free supernatants after 24 hours (nanograms per microgram of DNA). Results are expressed as the mean ± SEM of five independent experiments. *P < .05 compared with control cells. #P< .05 compared with AGP synthesis without anti-OSM.