Effect of IFN-γ on HIV infection of MDM. (A) Recombinant luciferase-encoding HIV reporter viruses pseudotyped with ADA Env were used to infect untreated MDM and IFN-γ–pretreated MDM (500 U/mL for 24 hours). Luciferase activity was quantitated in cell lysates 72 hours after infection. Results are representative of 4 (3 cord and 1 adult) experiments (*P < .0001, IFN-γv untreated). (B) Untreated or IFN-γ–pretreated (500 U/mL for 24 hours) MDM were infected with BaL strain of HIV and reverse transcriptase activity was measured in the culture supernatants at the times indicated. Each experiment was performed in triplicate and variability between triplicate results was less than 15%. Representative results from 4 donors (3 cord and 1 adult) are shown.