Fig. 1.
Inhibition of complement deposition on solid-phase bound rabbit aIgG by IVIG I. Dose response curves of the effect of IVIgM and IVIgG on in vitro complement deposition. Human serum diluted 1:200 in GVB++ was incubated for 1 hour at 37°C in aIgG-coated wells together with a serial dilution of IVIgM or IVIgG. Solid-phase bound C1q, C4, and C3 were then detected with specific DIG-labeled goat antibodies, sheep F(ab′)2 anti-DIG HRP conjugate and ABTS substrate. A representative experiment of three with similar results is shown.
