Effect of Dex on cell surface Muc-1 expression. RPMI 8226 MM cells, S6B45 MM cells, and DU145 prostate cancer cells were cultured in media alone or with Dex (10⁻⁸ mol/L) for 24 hours. Muc-1 expression was assessed by flow cytometry by staining with the VU-4H5 and VU-3C6 MoAbs relative to isotype control MoAbs. Peaks shown are denoted as follows: (*) staining with isotype control MoAb; (**) staining of cells cultured in media alone using either the VU-4H5 or the VU-3C6 MoAbs; (***) staining of cells cultured with Dex using either the VU-4H5 or the VU-3C6 MoAbs. No change in isotype control MoAb staining was seen with Dex stimulation.