Construction of deletion-type c-kit cDNA. (A) Schematic representation of deletion-type KIT. Location of signal peptide (SP), transmembrane (TM), and tyrosine residue of autophosphorylation (Y) are indicated. KIT^Tyr814 and KIT^Del-Tyr814 carries a point mutation (Asp to Tyr) in codon 814. (B) Expression of KIT^Del-Wild and KIT^Del-Tyr814. 293T cells were transfected with c-kit^Del-Wild and c-kit^Del-Tyr814 cDNA, and then cells were labeled for 5 hours with [³⁵S]-methionine and lysed. KIT was immunoprecipitated with ACK2 MoAb (ECD), rabbit anti-KIT^Kinase serum (Kinase), and rabbit anti-KIT^C-terminal serum (C-terminal) and analyzed by SDS-PAGE and autoradiography. The similar results were obtained from three independent experiments.