HPLC profile of products formed after incubation of a mixture of platelets and neutrophils with A23187 and PMA. Platelets (2 × 10⁸/mL) and neutrophils (5 × 10⁶/mL) were incubated for 30 minutes at 37°C with A23187 (5 μmol/L) and PMA (30 nmol/L). After addition of the internal standard (200 ng PGB₂), the products were analyzed by precolumn extraction/RP-HPLC as described in Materials and Methods. Abbreviations: B₄, LTB₄; t-B₄, 6-trans-LTB₄; 12e-t-B₄, 12-epi-6-trans-LTB₄; 5o-12h-ETE, 5-oxo-12-HETE; 8t-5o-12h-ETE, 8-trans-5-oxo-12(RS)-HETE.