American Society of Hematology

Fig. 2.

$Fig. 2. Binding of IIbβ3 and IIbD224Vβ3 to a GRGDSPK-Sepharose 4B column. Lysates of CHO cells stably transfected with the indicated receptors were loaded onto a 1 mL GRGDSPK-Sepharose column. The column was washed then eluted with 1 mmol/L GRGDS peptide (3 mL). One-milliliter column fractions were collected, resolved on a 8% nonreducing polyacrylamide gel, transfered onto nitrocelluose, and immunoblotted with the IIb-specific MoAb 98DF6 (3.5 μg/mL). L, lysate; FT, flow through; CE, RGD eluted fraction.$

Binding of _IIbβ₃ and _IIbD224Vβ₃ to a GRGDSPK-Sepharose 4B column. Lysates of CHO cells stably transfected with the indicated receptors were loaded onto a 1 mL GRGDSPK-Sepharose column. The column was washed then eluted with 1 mmol/L GRGDS peptide (3 mL). One-milliliter column fractions were collected, resolved on a 8% nonreducing polyacrylamide gel, transfered onto nitrocelluose, and immunoblotted with the _IIb-specific MoAb 98DF6 (3.5 μg/mL). L, lysate; FT, flow through; CE, RGD eluted fraction.

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