American Society of Hematology

Fig. 4.

$Fig. 4. Phenotype of hematopoietic cells differentiated from the VE-cadherin+ 4-integrin+ and VE-cadherin− 4-integrin+ cell fractions. The two fractions were sorted from differentiating CCE cells as shown in Fig 3A and cultured in a type IV collagen-coated dish in the presence of IL-3, Epo, G-CSF, and MGF for 7 days. (A through C) May-Gruenwald Giemsa staining of cytospots prepared from the cultured cells initiated from the VE-cadherin+4-integrin+ fraction. Erythroblasts (A), monocytes/macrophages (B), and polymorphonuclear cells (C) are observed. The bars represent 25 μm. (D and E) Expression of Mac-1 and TER119 on the cultured cells initiated from the VE-cadherin+ 4-integrin+ (D) and VE-cadherin− 4-integrin+ (E) cell fractions analyzed by flow cytometry. The numbers indicate the percentage of cells that appeared in each quadrant. The result shown is a representative of two independent experiments.$

Phenotype of hematopoietic cells differentiated from the VE-cadherin⁺ 4-integrin⁺ and VE-cadherin⁻ 4-integrin⁺ cell fractions. The two fractions were sorted from differentiating CCE cells as shown in Fig 3A and cultured in a type IV collagen-coated dish in the presence of IL-3, Epo, G-CSF, and MGF for 7 days. (A through C) May-Gruenwald Giemsa staining of cytospots prepared from the cultured cells initiated from the VE-cadherin⁺4-integrin⁺ fraction. Erythroblasts (A), monocytes/macrophages (B), and polymorphonuclear cells (C) are observed. The bars represent 25 μm. (D and E) Expression of Mac-1 and TER119 on the cultured cells initiated from the VE-cadherin⁺ 4-integrin⁺ (D) and VE-cadherin⁻ 4-integrin⁺ (E) cell fractions analyzed by flow cytometry. The numbers indicate the percentage of cells that appeared in each quadrant. The result shown is a representative of two independent experiments.

This Feature Is Available To Subscribers Only