Expression of VE-cadherin and 4-integrin on the cells dissociated from 9.5 dpc mouse embryos. The yolk sacs and embryonic bodies (lower trunk) were separated from 9.5 dpc mouse embryos and a single-cell suspension was prepared by using dispase and cell dissociation buffer. Cells were analyzed for expression of VE-cadherin and 4-integrin by flow cytometry (A). CD45⁺ cells and TER119⁺ cells were excluded from the analyses. The VE-cadherin⁺ 4-integrin⁻, VE-cadherin⁺ 4-integrin⁺, and VE-cadherin⁻ 4-integrin⁺ cells were sorted and reanalyzed (B). The numbers indicate the percentage of cells that appeared in each quadrant. The result shown is a representative of three independent experiments.