Stimulation of autologous, antileukemic cytotoxicity by fresh AML cells, AML-DC cultures, and IL-2 alone. Autologous T cells were cocultured with fresh AML PBMNC, AML-DC–rich cultures, or IL-2 alone as described in Materials and Methods. They were then tested for cytotoxicity against autologous leukemia targets by LDH-release assay. Results represent the mean cytotoxicity of triplicate cultures. (▪) AML-DC–stimulated T cells, (•) fresh AML PBMNC stimulated T cells, (○) T cells cultured in IL-2 alone. Cytotoxicity of DC-AL compared with IL-2–stimulated T cells at 25:1 ratio is significant (P< .005, Student’s t-test) for each patient. Cytotoxicity of fresh AML cell-stimulated T cells compared with IL-2–stimulated T cells at 25:1 ratio is significant (P < .010, Student’st-test) for patient 13.