TGF-β1 enhanced differentiation of CD11b^+hiCD11c⁻ cells from murine Lin⁻c-kit⁺ HPCs in the presence of GM-CSF + SCF with or without TNF at day 6. (A) Dose-dependent induction of CD11b^+hiCD11c⁻ cells by TGF-β1 in the cultures stimulated with GM-CSF + SCF + TNF. The data represent the mean value ± SD of the percentage of CD11b^+hiCD11c⁻ cells observed in more than five experiments. *P < .05 significance as compared with the cultures in the absence of TGF-β1. (B) Induction of CD11b^−/dullCD11c⁺, CD11b^+hiCD11c⁺, and CD11b^+hiCD11c⁻ cells by TGF-β1 in Lin⁻c-kit⁺ HPC cultures stimulated by various combination of cytokines as indicated. Quads were set up on the isotype-matched control dot plot, and the results are representative of more than five experiments.