TGF-β1 inhibited at day 12 the expression of Ia and CD86 antigens on CD11b^−/dullCD11c⁺ cells derived from cultured Lin⁻c-kit⁺ HPCs stimulated with the indicated various combinations of cytokines. Histograms shown in the figures were gated on CD11b^−/dullCD11c⁺ cells. Solid and dotted lines indicate the immunofluoresecence intensity of cells stained with a control and the test antibodies, respectively. Representative results from three or more independent experiments are shown.