Fig. 10.
Fig. 10. The effect of TGF-β1 on the expression of E-cadherin antigen and CCR7 mRNA in cultured Lin−c-kit+ HPCs. (A) Lin−c-kit+ HPCs were cultured in the presence of various combinations of cytokines as indicated for 12 days, followed by washing twice, and were recultured in the presence of GM-CSF + TNF for an additional 3 to 5 days. These recultured cells were processed for two-color analyses by staining with a rat-antimouse E-cadherin MoAb and a PE-labeled mouse antimouse Ia and finally shown by FITC-conjugated goat-antirat IgG(Fab′)2. Histograms shown in the figures were gated on Ia+hicells. Solid and dotted lines indicate the immunofluoresecence intensity of cells stained with a control MoAb and anti–E-cadherin antigen, respectively. Representative results from three experiments are shown. (B) Examination by RT-PCR of CCR7 mRNA in Lin−c-kit+ HPCs stimulated with indicated various combinations of cytokines at day 6 or 12.

The effect of TGF-β1 on the expression of E-cadherin antigen and CCR7 mRNA in cultured Lin−c-kit+ HPCs. (A) Lin−c-kit+ HPCs were cultured in the presence of various combinations of cytokines as indicated for 12 days, followed by washing twice, and were recultured in the presence of GM-CSF + TNF for an additional 3 to 5 days. These recultured cells were processed for two-color analyses by staining with a rat-antimouse E-cadherin MoAb and a PE-labeled mouse antimouse Ia and finally shown by FITC-conjugated goat-antirat IgG(Fab′)2. Histograms shown in the figures were gated on Ia+hicells. Solid and dotted lines indicate the immunofluoresecence intensity of cells stained with a control MoAb and anti–E-cadherin antigen, respectively. Representative results from three experiments are shown. (B) Examination by RT-PCR of CCR7 mRNA in Lin−c-kit+ HPCs stimulated with indicated various combinations of cytokines at day 6 or 12.

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