Purification of LAI. (A) Shows chromatography of HL-60 CM on Phenyl-Sepharose (1/40 similar chromatograms). Protein concentration is shown as absorbance at 280 nm; gradient 1 is shown as the left part of the dotted line and was measured as conductivity (C), and gradient 2 is the right part of the dotted line registered as percentage ethylene glycol (EG%). The insert shows percentage of CFU-GM in S-phase. (B) Shows ion exchange chromatography on MonoQ FPLC (1/22 similar). The gradient of increasing NaCl is shown as a dotted line and three regions of material that was pooled are shown (p1-3). Other symbols as in (A).