Fig. 3.
![Fig. 3. Detection of cell surface sialoadhesin lectin activity on Mm1 cells using a multivalent 3′-sialylactosylated probe (3′-PAA-FITC). Cultured Mm1 cells, a murine macrophage cell line, were washed, untreated, Arthrobacter Ureafaciens(AU)/VC-sialidase treated, or AU/VC-sialidase treated followed by preincubation with the 3D6 adhesion-blocking MoAb, and stained with FITC-conjugated 3′-PAA. Staining was detected using single color flow cytometry (fluorescence-activated cell sorting [FACS] analysis).](https://ash.silverchair-cdn.com/ash/content_public/journal/blood/93/4/10.1182_blood.v93.4.1245/5/blod40409003x.jpeg?Expires=1735536328&Signature=4YZLBsDXjZpm-h2mEQNozvqc48uEiJ7wCvEHr3bJFy2y7hkRklOOYdkIIggx6els1DRYAzR30e5rfc5-6FcNIq-b5pNElM1CIbWE8bXAKM9hkUcsf9XvHfQQKRdj9n0l3XrPPwpuPEjMoCqAXCK3jmqZtRvHHasvT3yP7r5jVRm0DtYvbP3V9-GOD7GAyo-iN-BGGhd01dcWWkJOrA6npQsngDeLc92nUJGTUcczq0qyXlEXMy8OBvep~QnhRPeyE5~vUMQIrgc9BhZeK4RsZuwRxKW~1whYPrsmHJHY-gWDrprB02MHzUlbXW~B-8CKlGOuzSx078fjMCDWHMn8rw__&Key-Pair-Id=APKAIE5G5CRDK6RD3PGA)
Detection of cell surface sialoadhesin lectin activity on Mm1 cells using a multivalent 3′-sialylactosylated probe (3′-PAA-FITC). Cultured Mm1 cells, a murine macrophage cell line, were washed, untreated, Arthrobacter Ureafaciens(AU)/VC-sialidase treated, or AU/VC-sialidase treated followed by preincubation with the 3D6 adhesion-blocking MoAb, and stained with FITC-conjugated 3′-PAA. Staining was detected using single color flow cytometry (fluorescence-activated cell sorting [FACS] analysis).
